That Big Rig You're Passing Might Just Be Full of Bees

Jalopnik By Andrew P. Collins June 25, 2019

Illustration: GMG Art Department/Peter Nelson (The Pollinators)

Illustration: GMG Art Department/Peter Nelson (The Pollinators)

There are still cowboys driving livestock across America in 2019. While most of us are snoozing, they’re rolling up to dark fields with trucks full of creatures that are critical to our nation’s agriculture: thousands and thousands of bees.

“Very few people know that this happens, and it happens as a necessity of the way our agriculture’s done,” Apiarist and filmmaker Peter Nelson explained to me. “I see bee trucks when I’m on the road, but most people don’t recognize them because it looks like a truck with boxes covered by a net.”

Nelson’s new movie The Pollinators is all about the bee industry, its huge role in our food system and the dire situation it’s in today. After months embedded with beekeepers documenting the complicated logistics of hauling bees from one end of the country to another, and years raising bees in his own backyard, he’s become something of an authority on the subject.

After watching his film myself, I have a whole new appreciation for this fascinating biological and economic ecosystem. I will now impart some of this wonder to you, before getting back to the part about trucks filled with bees driving down the highway at night.

Bees: We Fear Them, But We Must Love Them (Or We Starve)

Crops that make some of our favorite foods—almonds, broccoli, blueberries, avocados, apples—all need to be pollinated, and they’re pollinated by bees. But it takes armies of the insects to tend the immense commercial farms that get those foods to grocery stores. Since pollination only happens in certain seasons, it’s not practical for most farmers to stock and feed their own bees year-round. There definitely aren’t enough wild bees to get it done. And that’s why we’ve got a bee industry.

Photo: Peter Nelson ( The Pollinators )

Photo: Peter Nelson (The Pollinators)

Wild and farm-raised bees have slightly different lifestyles, but they have a lot of the same problems. Wild bees have to contend with their feeding grounds being paved and plowed for crops they can’t eat. Bees that work for humans for a living are at risk of being poisoned by pesticides designed to protect the plants that the bees are hired to pollinate. And all of them can succumb to parasitic varroa mites. These are tiny bugs that ride on bees and drink their blood, identified by the USDA as one of a bee’s biggest threats.

The importance of both natural and commercial pollination is well documented, as are the threats to their systems. If you want to dive deeper into the science of the situation, The Center for Biological Diversity’s paper Pollinators In Peril from 2017 might be a good place to start.

More recently, the plight of pollinators is starting to sneak its way into pop culture. Even PornHub is using its platform to make people realize how important bees are. But to truly appreciate what’s happening, you’ve got to wrap your head around the scale and significance of the bee industry.

Why Are We Trucking Bees Around, Exactly?

There are more than 90 million almond trees in California. They need to be pollinated every year, and it takes over 31 billion bees to make that happen.

Since there aren’t enough natural pollinators to take care of today’s commercial crops, just like there’s not enough rain to water them without the help of irrigation, the rental bees are brought in. Those same bees could get booked in every other corner of the country too, pollinating different crops in different seasons.

Photo: Peter Nelson ( The Pollinators )

Photo: Peter Nelson (The Pollinators)

After almond season, the bees might get shipped up to the pacific northwest for apples. Then Massachusetts for cranberries, Maine for blueberries, or, some go to North and South Dakota to relax and focus on making honey, as highlighted by an article in The Conversation that notes many beekeepers are based there.

Nelson told me there are approximately 2,000 beekeepers with “more than 300 hives,” which he also explained was about the threshold from where a hobbyist or sideliner beekeeper becomes a serious commercial player. “The biggest beekeeper in the country is about 100,000 hives,” he added.

And how many bees does that entail? The typical hives Nelson had seen tended to house about 25,0000 bees. But bee colonies expand and contract over the course of a year. An Oregon State University paper cited by GrowOrganic stated that you could have between 10,000 and 60,000 bees living together.

If bees are comfortable, they can multiply fast. A typical worker bee only lives for about 40 days, but population growth can be fast in the right conditions. A queen can lay up to 1,500 eggs a day when the environment is optimized. So a beekeeper renting hives to farmers could be working with many generations of the insects, at the same hive, over the course of a year.

Photo: Peter Nelson ( The Pollinators )

Photo: Peter Nelson (The Pollinators)

Today beekeepers at big scale make most of their money from pollination services, as opposed to 20 years ago, Nelson told me, when honey production was more lucrative. But as farms expanded and natural bee habitats have contracted, the demand for rental bees has gone up.

Pollination fees vary. “Last year ranged from $175 to $225 per hive for almond pollination,” said Nelson. “And that’s the biggest pollination in the country. Honeybees are essential to almonds, so they command a higher price.”

Almond crops need two hives per acre to be pollinated completely, so the dollar figure starts to swell pretty quickly. The Center for Biological Diversity says “more than $3 billion dollars” changes hands for fruit-pollination services in the U.S. every year.

And yet, sometimes commercial beekeeping business relationships are pretty old-school. “a lot of these contracts or agreements are made on a handshake,” Nelson explained. “Dave Hackenberg, [well-known professional beekeeper, credited as the first to raise awareness about colony collapse disorder] he’s been keeping bees my whole life, and he has some of his regular clients that go back 30 years.”

OK, So Here’s How You Haul Bees

Bees are considered livestock, so people charged with moving them are supposed to be comfortable working with animals. Still, transporting bees presents some unique challenges. Like, if you stop on a warm day, your cargo might just buzz away. That, or get trapped in the net covering the truck’s cargo deck, and that’s just a bad day for everybody.

Photo: Peter Nelson ( The Pollinators )

Photo: Peter Nelson (The Pollinators)

If a bee truck crashes, “it’s a mess,” Nelson told me. It happens, and when it does, beekeepers will try to save their wares. If the queen bee stays in the hive, which they normally do, the rest of the bees will buzz back. But the insects can only get back to their hive if it’s in the same place they left it. If a cleanup crew has to move the hives, or replace them, rounding up all the loose bees can be impossible.

Most bee hauling runs don’t end in that kind of disaster, but they are a lot of work. Let’s say a big rig’s worth of bees need to get from Georgia to California early in the year, for the start of the almond season.

Bees are generally loaded up for transportation at nighttime. That’s partially because the cold slows them down, but mainly on account of that geolocation phenomenon I just mentioned. If the bees go to bed in one place and wake up in another, they apparently don’t care, and go about their business pollinating in the new spot. But if you move the hive while the bees are active, they get confused and lost.

Photo: Peter Nelson ( The Pollinators )

Photo: Peter Nelson (The Pollinators)

Before being heaved onto the deck of an empty flatbed trailer, bees are often calmed down with smoke. (Bees: They’re just like us!) The trick is that beekeepers go around their hives with smoke-dispensing canisters to make the insects think there’s a forest fire.

You might imagine that would send them into an apocalyptic panic, but apparently it has the opposite effect. Kind of. The bees gorge themselves on honey, either in preparation for evacuation or just resignation that doomsday is near, and become significantly more docile than they usually are.

Smoke also “blocks pheromones and makes it harder for them to sting,” says Nelson.

With the bees toked out, about 400 to 425 palletized hives can be stacked onto a semi-truck trailer with a forklift. Multiply that by 25,000 bees per hive, and yeah, you could have more than 10,000,000 on a truck easily.

Once the bees are rolling, their humans like to keep them in motion as much as possible during the day since the wind discourages them from going outside. If beekeepers do have to stop, they try to do it at high elevation where it’s cooler and bees will be more motivated to stay indoors. Once again, I am realizing how bee-like my own existence is... I don’t like to leave the house unless the weather’s soft, either. Also, if my house moved I would definitely get lost.

Photo: Peter Nelson (The Pollinators)

Photo: Peter Nelson (The Pollinators)

Speaking of weather, that’s the last, and most critical, factor bee haulers have to worry about. If it’s too warm, the bees will escape and die. If it’s too cold, the bees will die. If it rains or snows, that presents its own set of problems.

“The thing that they’re all watching is weather... The almond pollination, that’s probably the riskiest one,” Nelson explained, “because a lot of the distances they cover are long. From Florida, Georgia, Alabama, all the way to Central Valley, California. And then also because of the weather that time of year (January and February) is a little more volatile.”

Beekeepers will even pre-run their hauling routes, just like Baja racers, to scout good spots to stop and plan their pacing. “A lot of beekeepers will go these exact routes beforehand,” Nelson added, “so they’ll know places where, ‘OK if you need to pull off, this is a good place, because it has an elevation that’s a little bit higher, so it might be cooler and better for the bees to stay in the hive,’” for example.

A 2018 Agweek article cited Miller Honey Farms Vice President Jason Miller as stating his companies hives “lose about two percent of their bees each time they’re moved,” and also mentioned that bee farmers sometimes have to get creative when it comes to finding places to park the bees in down time. The Miller operation apparently rents potato cellars in Idaho as their bees’ winter home.

Photo: Peter Nelson ( The Pollinators )

Photo: Peter Nelson (The Pollinators)

But even if beekeepers manage to keep their bees alive through the cold months, and get them on-and-off trucks safely, they still have to deal with bee bandits.

Yes, beehive theft is a thing. National Geographic recently reported that $70,000 worth of buzzing gold (bees) was heisted from a California farm. In 2016, somebody made off with $200,000 worth of bees in Canada and similar crimes have happened in England, New Zealand and elsewhere.

In the U.S., California’s Rural Crime Prevention Task Force deals with this kind of thing. “These cases are hard to crack because bees don’t have VIN numbers like cars, and we can’t track them by their DNA,” Detective Isaac Torres of the Task Force is quoted saying in that Nat Geo article. But stolen bees do get found, and the California State Beekeepers Association apparently “offers a $10,000 reward for information resulting in the arrest and conviction of a bee rustler.”

How Do We Befriend The Bees, And Earn Their Trust And Respect?

If you’ve read this far, you’ve got an understanding of how hard bees and their keepers have it. Some even say forcing bees to work for us at all is exploitive and wrong. But short of trying to topple the bee industry, it is possible for people to proactively be part of a pollination solution.

Photo: Peter Nelson ( The Pollinators )

Photo: Peter Nelson (The Pollinators)

“One of things I like to suggest to people is to support their local beekeepers,” Nelson told me when I asked him how I could help the bees. “Buy[ing] honey locally, certainly buy[ing] U.S. honey,” helps our bee economy, but planning a garden that’s pollinator-friendly if you have the space for it, and minimizing the use of pesticides around your house goes a long way too.

Not all pollinating bees are honeybees that can fly five miles or get carted thousands of miles across the country. Some local pollinators might just hang out in your yard.

Making good food choices, as in buying food that’s pollinated sustainably, can be difficult to do. It’s a very positive step in helping the environment, though. And now that you know that, you might have some more research to do. But at least, next time you see a truck with stacks of boxes covered by a net, you’ll know what it’s up to!

For a longer look at the life of bees on the road and the people making a lot of your food happen, you really should try to see The Pollinators movie, which you might be able to catch at a film festival soon.

https://jalopnik.com/that-big-rig-youre-passing-might-be-full-of-bees-1834383949

Quantum Dots Track Pollinators

The Optical Society By Stewart Wills February 18, 2019

A bee, caught after visiting a flower whose pollen grains had been labelled with quantum dots. The glowing dots show evidence of the bee’s travels, and how the pollen attaches to it, when the insect is examined under the microscope in UV light. [Image: Corneile Minnaar]

A bee, caught after visiting a flower whose pollen grains had been labelled with quantum dots. The glowing dots show evidence of the bee’s travels, and how the pollen attaches to it, when the insect is examined under the microscope in UV light. [Image: Corneile Minnaar]

A particularly sobering aspect of global environmental degradation is the rapid decline of insect populations. One recent study in the journal Biological Conservation estimated that 40 percent of the world’s insect species could go extinct within the next three decades, owing to habitat loss due to agriculture and urbanization, pesticides, climate change and other insults.

Quite apart from playing havoc with the food web, declines in certain insect populations threaten the bugs’ crucial role as pollinators. Humans rely on insects to pollinate more than 30 percent of food crops—a huge service that nature provides free of charge.

That makes it essential to understand which insects are pollinating which plants—even to the point of tracking individual pollen grains from flower to flower via their insect vectors. But a robust, useful system for labeling the tiny grains, which are subject to the vicissitudes of wind and weather in addition to the mazy paths of insects, has been fiendishly difficult to devise. Now, a pollination biologist in South Africa has hit upon a novel answer: tag the pollen with fluorescent quantum dots (Meth. Ecol. Evol., doi: 10.1111/2041-210X.13155).

Dots, flowers and pollen

Quantum dots (QDs) are luminescent semiconductor nanocrystals that, when excited by light of a specific wavelength (such as UV), re-emit at visible wavelengths, with the specific emission wavelength depending on the size of the quantum dot. They’ve found use in a wide variety of contexts including biomedical study (see, “Quantum Dots for Biomedicine,” OPN, April 2017). Indeed, the pollination biologist behind the new study, Corneile Minnaar of Stellenbosch University, South Africa, reportedly got the idea for pollen tracking with QDs from a paper on their potential use in targeting and imaging cancer cells.

Corneile Minnaar, applying a solution of lipid-tagged quantum dots to the business end of a flower. [Image: Ingrid Minnaar]

Corneile Minnaar, applying a solution of lipid-tagged quantum dots to the business end of a flower. [Image: Ingrid Minnaar]

To use QDs to track individual pollen grains, Minnaar—who began the work as a Ph.D. student at Stellenbosch, where he’s now a postdoc in the lab of pollination biologist Bruce Anderson—first had to figure out how to tie the dots to the pollen. To do so, he began with commercially available, nontoxic CuInSexS2−x/ZnS (core/shell) QDs with four different emission wavelengths: 550 nm (green), 590 nm (yellow), 620 nm (orange) and 650 nm (red). Next, Minnaar chemically tied the QDs to an oleic‐acid ligand molecule that would latch onto the lipid-rich “pollenkitt” that surrounds pollen grains—the same substance that makes pollen stick to the coats of pollinators like honeybees.

Minnaar then took the lipid-doped QDs and dissolved them into a volatile hexane solvent, and micro-pipetted drops of the solvent onto the pollen-rich anthers on flowers of four different plant species. The ligand-bearing QDs quickly stuck to the pollenkitt on the grains, as expected, and the volatile hexane rapidly evaporated away. The result: flowers packed with potentially trackable, QD-labeled pollen.

Building an “excitation box”

The next problem to be solved was how actually to read the signal from the tagged pollen. While Minnaar says he started with a toy pen with a UV LED light to excite the fluorescence in the dots, he clearly needed something a bit more scalable. To get there, he used a 3-D printer to create a black “quantum-dot excitation box” that could fit under a dissection microscope, and that included four commercial UV LEDs, a long-pass UV filter, and supporting housing. In a press release accompanying the work, Minnaar said the UV box could “easily be 3D-printed at a cost of about R5,000 [around US$360], including the required electronic components.”

Minnaar tested the ability of the pollen grains to hold onto the QDs by agitating samples in an ethanol solution, and found that the grip was firm. Also, in a controlled, caged experiment, he trained honeybees to move from tagged to untagged samples of a particular flower species, and found that labeling the grains with the QDs had no effect on the grains’ ability also to stick to the bees.

Robust system

The general robustness of the system suggests it could serve well in tracking pollinators in wild settings, quantifying parameters such as pollen loss and the importance of certain species to sustaining specific kinds of plants. That said, there are still a few limitations, according to the paper. One is that right now, “there are only four commercially available, distinguishable quantum dot colors in the visible range,” which could limit studies to only four plant species at a time. And, while initial tests were encouraging, more work needs to be done to determine whether the labeling and application process has effects on pollen viability that could complicate experiments or affect pollinator behavior.

One other, unavoidable drawback, notwithstanding Minnaar’s clever microscope setup, is the sheer labor of counting and checking the glowing pollen grains to amass experimental data. That's a task likely to while away the hours of grad students for years to come, irrespective of the technique used to label the grains. “I think I've probably counted more than a hundred thousand pollen grains these last three years,” Minnaar said.

Source: British Ecological Society

https://www.osa-opn.org/home/newsroom/2019/february/quantum_dots_track_pollinators/